Part:BBa_K1627002:Design

Device to demethylate 7-methylxanthine

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 498
  Illegal BglII site found at 994
  Illegal BglII site found at 1465
  Illegal XhoI site found at 2606
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3304
  Illegal AgeI site found at 1133
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.

Source

The ndmC and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.